The use of molecular techniques for bacterial detection in the analysis of gastric aspirates collected from infants on the first day of life (Record no. 75419)

MARC details
000 -LEADER
fixed length control field 02629cam a2200241 4500
001 - CONTROL NUMBER
control field NMDX5942
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 120401t xxu||||| |||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 03783782
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Jones, V.
240 ## - UNIFORM TITLE
Uniform title <a href="Early Human Development">Early Human Development</a>
245 ## - TITLE STATEMENT
Title The use of molecular techniques for bacterial detection in the analysis of gastric aspirates collected from infants on the first day of life
500 ## - GENERAL NOTE
General note NMUH Staff Publications
500 ## - GENERAL NOTE
General note 86
520 ## - SUMMARY, ETC.
Summary, etc. &lt;p class="MsoNoSpacing"&gt;&lt;span lang="EN-US"&gt;Prospective service evaluation of the utility of molecularmethods to analyse neonatal gastric aspirate specimens in a single neonatalunit and associated maternity unit. 43 newborn infants investigated for sepsiswith median gestational age of 39 weeks (range 31-41 weeks) and median birthweight 3050 grams (range 1250-4220 g). Gastric aspirates routinely collectedwithin 12h of birth were analysed using conventional and molecular methods for bacterialdetection, bacterial DNA load and sequencing to identified bacterial species.&lt;/span&gt;&lt;/p&gt;&lt;p class="MsoNoSpacing"&gt;&lt;span lang="EN-US"&gt;RESULTS: Bacterial DNA loads varied from 0.03 to 1736pg/microl of DNA extract (1 microl of DNA extract equivalent to 4 microl gastricaspirate). Bacteria were identified in 30/43 (70%) of samples by molecularmethods and 10/43 (23.3%) of samples by culture. Cultures were only positivewhen the bacterial DNA exceeded 4.5 pg/microl of extract. Infants withprolonged rupture of membranes (&amp;gt;24h prior to delivery) had a DNA load onaverage 23 times higher than those without (95%CI 3.7 to 141; p=0.001).Additional bacteria detected by molecular methods included many species thatare fastidious and potentially pathogenic including Leptotrichia spp., Serratiaspp., Ureaplasma spp., Veillonella spp., Haemophilus influenzae and Group BStreptococcus. Due to a low rate of adverse outcomes it was not possible tocorrelate bacterial identifications or DNA load with infant outcome.&lt;/span&gt;&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p class="MsoNoSpacing"&gt;&lt;span lang="EN-US"&gt;CONCLUSIONS: Molecular methods can identify bacteriafrom a greater proportion of gastric aspirate specimens that conventionalculture. Further work is required to establish whether this information can beused to improve infant outcomes.&lt;/span&gt;&lt;/p&gt;
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Wilks, M.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Johnson, G.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Warwick, S.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Hennessey, E.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Kempley, S.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Millar, M.
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://www.ncbi.nlm.nih.gov/pubmed/20223606">http://www.ncbi.nlm.nih.gov/pubmed/20223606</a>
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://ferriman.wufoo.com/forms/r7x3a7/">http://ferriman.wufoo.com/forms/r7x3a7/</a>
Holdings
Withdrawn status Lost status Damaged status Not for loan Collection code Home library Current library Shelving location Date acquired Total Checkouts Date last seen Price effective from Koha item type
        Staff publications for NMDX Ferriman information and Library Service (North Middlesex) Ferriman information and Library Service (North Middlesex) Shelves 07/06/2022   07/06/2022 07/06/2022 Book
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